CYP27B1 CLIA Kit for Human(Homo sapiens) | EIAab.comCYP27B1 CLIA Kit for Human(Homo sapiens)

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CYP27B1 (Gene name),

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (Protein name ), CP27B_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

CYP27B1(CYP1ALPHA;CYP27B);

Protein name:

25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial;

Alternative:

25-hydroxyvitamin D(3) 1-alpha-hydroxylase(VD3 1A hydroxylase);25-OHD-1 alpha-hydroxylase;Cytochrome P450 subfamily XXVIIB polypeptide 1;Calcidiol 1-monooxygenase;Cytochrome P450VD1-alpha;Cytochrome P450C1 alpha;Cytochrome p450 27B1;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

N/A

Function:

Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.

Subcellular Location:

Mitochondrion membrane

Protein Attributes:

Sequence length:

508

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

508:

INLQFLDR

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

MIM:

264700

STRING:

9606.ENSP00000228606

KEGG:

hsa:1594

Pfam:

PF00067

UniGene:

Hs.524528

String:

O15528

SMR:

O15528

Uniprot:

O15528

FOR

ELISA Kit for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Cat.:

E10324h

Price:

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Manual:

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MSDS:

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Packing:

96T

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ELISA Kit for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Cat.:

E10324r

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Manual:

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Packing:

96T

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ELISA Kit for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Cat.:

E10324m

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MSDS:

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Packing:

96T

CLIA Kit for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Cat.:

U10324r

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Cat.:

U10324m

Price:

Please sign in first.

MSDS:

Please sign in first.

Packing:

96T

CLIA Kit for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Cat.:

U10324h

Price:

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MSDS:

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Packing:

96T

Polyclonal Antibody for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Cat.:

P10324Rb-h

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Monoclonal Antibody for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Protein for Human 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.9%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

Recovery was determined by spiking various levels of CP21B into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	92	89-103
Plasma	93	86-102

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CP21B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	87-97%	85-101%	83-103%	86-97%
EDTA plasma(n=5)	89-98%	87-99%	89-96%	90-95%
heparin plasma(n=5)	85-100%	86-101%	84-102%	88-108%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

References

1.

"Complete structure of the human gene for the vitamin D 1alpha-hydroxylase, P450c1alpha."
Fu G.K. , Portale A.P. , Miller W.L.
DNA Cell Biol.16:1499-1507(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
2.

"Molecular cloning of cDNA and genomic DNA for human 25-hydroxyvitamin D3 1 alpha-hydroxylase."
Monkawa T. , Yoshida T. , Wakino S. , Shinki T. , Anazawa H. , Deluca H.F. , Suda T. , Hayashi M. , Saruta T.
Biochem. Biophys. Res. Commun.239:527-533(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]
tissue: Kidney.
3.

"Cloning of human 25-hydroxyvitamin D-1 alpha-hydroxylase and mutations causing vitamin D-dependent rickets type 1."
Fu G.K. , Lin D. , Zhang Y.H. , Bikle D.D. , Shackleton C.H. , Miller W.L. , Portale A.A.
Mol. Endocrinol.11:1961-1970(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
4.

"Targeted disruption of the 25-hydroxyvitamin D3 1alpha-hydroxylase gene in ras-transformed keratinocytes demonstrates that locally produced 1alpha,25-dihydroxyvitamin D3 suppresses growth and induces differentiation in an autocrine fashion."
Huang D.C. , Papavasiliou V. , Rhim J.S. , Horst R.L. , Kremer R.
Mol. Cancer Res.1:56-67(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]
5.

"Targeted disruption of the 25-hydroxyvitamin D3 1 a-hydroxylase gene in a Ras-transformed human keratinocyte cell line: evidence for an autocrine growth regulatory function of 1 alpha, 25-dihydroxyvitamin D3 in vitro and in vivo."
Huang D.C. , Papavasiliou J. , Rhim J. , Kremer R.
Submitted (2000-03) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
6.

NIEHS SNPs program
Submitted (2003-04) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANT LEU-166
7.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
tissue: Kidney.
8.

Mural R.J. , Istrail S. , Sutton G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
9.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]
10.

"Inactivating mutations in the 25-hydroxyvitamin D3 1alpha-hydroxylase gene in patients with pseudovitamin D-deficiency rickets."
Kitanaka S. , Takeyama K. , Murayama A. , Sato T. , Okumura K. , Nogami M. , Hasegawa Y. , Niimi H. , Yanagisawa J. , Tanaka T. , Kato S.
N. Engl. J. Med.338:653-661(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS VDDR1A HIS-107; GLU-125; PRO-335 AND SER-382
11.

"Genetics of vitamin D 1-alpha-hydroxylase deficiency in 17 families."
Wang J.T. , Lin C.-J. , Burridge S.M. , Fu G.K. , Labuda M. , Portale A.A. , Miller W.L.
Am. J. Hum. Genet.63:1694-1702(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS VDDR1A HIS-65; LYS-189; HIS-389; ILE-409; PRO-429; CYS-453 AND ARG-497
12.

"Novel mutations in the 1alpha-hydroxylase (P450c1) gene in three families with pseudovitamin D-deficiency rickets resulting in loss of functional enzyme activity in blood-derived macrophages."
Smith S.J. , Rucka A.K. , Berry J.L. , Davies M. , Mylchreest S. , Paterson C.R. , Heath D.A. , Tassabehji M. , Read A.P. , Mee A.P. , Mawer E.B.
J. Bone Miner. Res.14:730-739(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS VDDR1A TYR-323 AND GLY-478
13.

"No enzyme activity of 25-hydroxyvitamin D3 1alpha-hydroxylase gene product in pseudovitamin D deficiency rickets, including that with mild clinical manifestation."
Kitanaka S. , Murayama A. , Sakaki T. , Inouye K. , Seino Y. , Fukumoto S. , Shima M. , Yukizane S. , Takayanagi M. , Niimi H. , Takeyama K. , Kato S.
J. Clin. Endocrinol. Metab.84:4111-4117(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS VDDR1A ARG-321 AND CYS-389
14.

"Novel gene mutations in patients with 1alpha-hydroxylase deficiency that confer partial enzyme activity in vitro."
Wang X. , Zhang M.Y. , Miller W.L. , Portale A.A.
J. Clin. Endocrinol. Metab.87:2424-2430(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: VARIANTS VDDR1A GLY-189; PHE-343; GLY-389; HIS-389 AND ILE-409

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